Three-Dimensional Cell Culture Assays: Are They More Predictive of In Vivo Efficacy than 2D Monolayer Cell-Based Assays?

Abstract

ASSAY and Drug Development TechnologiesVol. 13, No. 5 Roundtable DiscussionThree-Dimensional Cell Culture Assays: Are They More Predictive of In Vivo Efficacy than 2D Monolayer Cell-Based Assays?Moderator: Sitta Sittampalam, Participants: Richard Eglen, Stephen Ferguson, Jason Thomas Maynes, Kenneth Olden, Laura Schrader, Todd Shelper, and Marc FerrerModerator: Sitta SittampalamSenior Advisor to the Director, National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, Maryland.Search for more papers by this author, Participants: Richard EglenVice President and General Manager, Corning Life Sciences, Tewksbury, Massachusetts.Search for more papers by this author, Stephen FergusonChemist, Molecular Toxicology and Informatics Group, Biomolecular Screening, National Institute of Environmental Science, National Institutes of Health, Research Triangle Park, North Carolina.Search for more papers by this author, Jason Thomas MaynesDirector of Research, Anesthesia and Pain Medicine/Staff Anesthesiologist, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.Search for more papers by this author, Kenneth OldenDirector, National Center for Environmental Assessment, Environmental Protection Agency, Arlington, Virginia.Search for more papers by this author, Laura SchraderCEO, 3D Biomatrix, Ann Arbor, Michigan.Search for more papers by this author, Todd ShelperResearch Fellow, Avery Group, Eskitis Institute for Drug Discovery, Griffith University, Nathan, Queensland, Australia.Search for more papers by this author, and Marc FerrerLeader, Biomolecular Screening and Probe Development, Division of Pre-Clinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, Maryland.Search for more papers by this authorPublished Online:13 Jul 2015https://doi.org/10.1089/adt.2015.29001.rtdAboutSectionsView articleView Full TextPDF/EPUB ToolsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail View articleFiguresReferencesRelatedDetailsCited ByUsing 3D in vitro cell culture models in anti-cancer drug discovery26 April 2021 | Expert Opinion on Drug Discovery, Vol. 16, No. 8Precision Oncology Beyond Genomics: The Future Is Here—It Is Just Not Evently Distributed17 April 2021 | Cells, Vol. 10, No. 4Extracellular vesicles from organoids and 3D culture systems31 October 2020 | Biotechnology and Bioengineering, Vol. 118, No. 3Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology13 February 2021 | Nanomaterials, Vol. 11, No. 2TIRF Microscopy‐Based Monitoring of Drug Permeation Across a Lipid Membrane Supported on Mesoporous Silica25 November 2020 | Angewandte Chemie, Vol. 133, No. 4TIRF Microscopy‐Based Monitoring of Drug Permeation Across a Lipid Membrane Supported on Mesoporous Silica23 November 2020 | Angewandte Chemie International Edition, Vol. 60, No. 4Water-Templated, Polysaccharide-rich Bioartificial 3D Microarchitectures as Extra-Cellular Matrix Bioautomatons7 April 2020 | ACS Applied Materials & Interfaces, Vol. 12, No. 18Comparison of 2D and 3D cell cultures of colorectal adenocarcinoma as models for drug screening6 February 2020 | Russian Chemical Bulletin, Vol. 68, No. 12High throughput scaffold-based 3D micro-tumor array for efficient drug screening and chemosensitivity testingBiomaterials, Vol. 198Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning23 January 2018 | Frontiers in Pharmacology, Vol. 9A 96-well microplate bioreactor platform supporting individual dual perfusion and high-throughput assessment of simple or biofabricated 3D tissue models1 January 2018 | Lab on a Chip, Vol. 18, No. 18Personalized Medicine8 December 2017A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes8 May 2017 | Biotechnology and Bioengineering, Vol. 114, No. 8Designing a bio-inspired biomimetic in vitro system for the optimization of ex vivo studies of pancreatic cancerDrug Discovery Today, Vol. 22, No. 4A semiautomated microfluidic platform for real-time investigation of nanoparticles’ cellular uptake and cancer cells’ trackingNanomedicine, Vol. 12, No. 6Screening out irrelevant cell-based models of disease12 September 2016 | Nature Reviews Drug Discovery, Vol. 15, No. 11Cancer drug discovery: recent innovative approaches to tumor modeling11 August 2016 | Expert Opinion on Drug Discovery, Vol. 11, No. 9State-of-the-Art Metabolic Toxicity Screening and Pathway Evaluation14 April 2016 | Analytical Chemistry, Vol. 88, No. 9 Volume 13Issue 5Jun 2015 InformationCopyright 2015, Mary Ann Liebert, Inc.To cite this article:Moderator:, Sitta Sittampalam, Participants:, Richard Eglen, Stephen Ferguson, Jason Thomas Maynes, Kenneth Olden, Laura Schrader, Todd Shelper, and Marc Ferrer.Three-Dimensional Cell Culture Assays: Are They More Predictive of In Vivo Efficacy than 2D Monolayer Cell-Based Assays?.ASSAY and Drug Development Technologies.Jun 2015.254-261.http://doi.org/10.1089/adt.2015.29001.rtdPublished in Volume: 13 Issue 5: July 13, 2015Online Ahead of Print:June 29, 2015PDF download