Abstract
Three-dimensional (3D) cell culture is attracting increasing attention today because it can mimic tissue environments and provide more realistic results than do conventional cell cultures. On the other hand, very little attention has been given to using 3D cell cultures in the field of avian cell biology. Although mimicking the bone marrow niche is a classic challenge of mammalian stem cell research, experiments have never been conducted in poultry on preparing in vitro the bone marrow niche. It is well known, however, that all diseases cause immunosuppression and target immune cells and their development. Hematopoietic stem cells (HSC) reside in the bone marrow and constitute a source for immune cells of lymphoid and myeloid origins. Disease prevention and control in poultry are facing new challenges, such as greater use of alternative breeding systems and expanding production of eggs and chicken meat in developing countries. Moreover, the COVID-19 pandemic will draw greater attention to the importance of disease management in poultry because poultry constitutes a rich source of zoonotic diseases. For these reasons, and because they will lead to a better understanding of disease pathogenesis, in vivo HSC niches for studying disease pathogenesis can be valuable tools for developing more effective disease prevention, diagnosis, and control. The main goal of this review is to summarize knowledge about avian hematopoietic cells, HSC niches, avian immunosuppressive diseases, and isolation of HSC, and the main part of the review is dedicated to using 3D cell cultures and their possible use for studying disease pathogenesis with practical examples. Therefore, this review can serve as a practical guide to support further preparation of 3D avian HSC niches to study the pathogenesis of avian diseases.
Highlights
As a precursor of immune cells, hematopoietic stem cells (HSC) play an indispensable role in the immune response against the main avian diseases (Cui et al, 2009; Gurung et al, 2017; Hosokawa et al, 2020)
HSC reside in a unique bone marrow environment, where they interact with other cells and molecules to create a bone marrow niche (Zhang P. et al, 2019)
Standard morphological viability assays can be performed by fluorescence microscopy using confocal laser scanning microscopy (CLSM)
Summary
As a precursor of immune cells, hematopoietic stem cells (HSC) play an indispensable role in the immune response against the main avian diseases (Cui et al, 2009; Gurung et al, 2017; Hosokawa et al, 2020). Current approaches in mimicking bone marrow environments comprise scaffold-based systems using hydrogels, and macroporous and nanofiber scaffolds (Bello et al, 2018). These scaffolds can be embedded in perfused chambers and through microfluidic technology, further enhancing the biocompatibility of cell culture (Bhatia and Ingber, 2014). In vitro studies constitute the gold standard for researching disease pathogenesis; it is interesting that there exists a lack of studies involving pathogens’ interactions with HSC to evaluate impacts of disease causative agents on the fate and differentiation of HSC and on immune cell development. The main goal of this review, is to provide information about 3D cell cultures that will be useful for in vivo avian HSC 3D culture preparation and further conceiving of host–pathogen studies
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