Three CuII and CoII Coordination Complexes Containing Tridentate Schiff Base Moieties Induce ROS Generation and Lead to Caspase-Dependent Apoptotic Cell Death in Intracranial Aneurysm — RETRACTED

Abstract

Two similar tridentate Schiff base ligands with N,O,O-donor sites, 2-methoxy-6-(((2-(piperazin-1-yl)ethyl)amino)methyl)phenol (HL1) and 2-(((2-(dimethylamino)ethyl)amino)methyl)-6-methoxyphenol (HL2) have been synthesised by a one pot condensation reaction, and were further used in the construction of three novel Schiff base CuII and CoII coordination complexes [Cu(L1)(OAc)] (1), [Co(L2)(OAc)] (2), and [Cu(L2)(N3)(MeOH)] (3). Furthermore, the particle sizes of these coordination complexes have been successfully reduced to the nano-region via a hand grinding method. In addition, the anti-proliferation activity of nano 1–3 was detected on human intracranial aneurysm SF767 cells with a Cell Counting Kit-8 assay. The half maximal inhibitory concentration values calculated from the cell viability curves indicated that only nano 1 has anti-proliferation activity on SF767 cells. To further investigate the anticancer mechanism of nano 1, a Western blot assay, reactive oxygen species level detection, and an Annexin V-FITC/PI double staining assay were conducted.