Abstract
As a folk medicine, Moringa oleifera L. is used effectively to treat inflammatory conditions and skin diseases. However, its mechanism of action is not well understood, limiting its medical use. We isolated and identified three compounds, namely niazirin, marumoside A and sitosterol-3-O-β-d-glucoside, from the seeds of Moringa oleifera, and studied their effects on the expression of Th17-relevant cytokines (IL-12/IL-23 p40, IL-17A, IL-22 and IL-23 p19) using lipopolysaccharide-stimulated THP-1 cells. Additionally, as Th17 plays a critical role in the pathogenesis of psoriasis, we used a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced psoriasis-like skin lesion mouse model to study their potential therapeutic application in vivo. The compounds suppressed the expression of IL-12/IL-23 p40, IL-17A, IL-22 and IL-23 p19 in vitro, and in vivo they ameliorated psoriasis-like skin lesions, decreased IL-17A mRNA expression, and increased the expression of keratinocyte differentiation markers. To our knowledge, this is the first report regarding the mechanism and therapeutic application of Moringa oleifera seeds to treat psoriasis-like lesions in vivo.
Highlights
Moringa oleifera L. (M. oleifera), known as horseradish tree, drumstick tree, benzolive tree or ben oil tree, is widely cultivated in Africa, tropical Asia, Latin America and the Pacific Islands [1].The plant is well known for its industrial and traditional medicine uses, including antibiosis or malaria treatment and treatment of typhoid fever, parasitic diseases, genitourinary ailments, hypertension, inflammatory diseases, swellings, skin diseases, hypoglycemia and diabetes [1,2,3,4,5,6]
Considering that Th17-relevant cytokines are critically involved in the pathogenesis of psoriasis [14], we further evaluated their potential therapeutic application in psoriasis using a 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated mouse model
Three constituents were purified from M. oleifera with purities greater than 90% determined by high performance liquid chromatography (HPLC)
Summary
Moringa oleifera L. (M. oleifera), known as horseradish tree, drumstick tree, benzolive tree or ben oil tree, is widely cultivated in Africa, tropical Asia, Latin America and the Pacific Islands [1].The plant is well known for its industrial and traditional medicine uses, including antibiosis or malaria treatment and treatment of typhoid fever, parasitic diseases, genitourinary ailments, hypertension, inflammatory diseases, swellings, skin diseases, hypoglycemia and diabetes [1,2,3,4,5,6]. Many studies have reported pharmacological properties of M. oleifera in vitro and in vivo, including antihypertensive, hepatoprotective, diuretic, cholesterol lowering, anti-inflammatory, antibacterial and antitumor activities [5,7,8,9]. The bioactive constituents and their anti-inflammatory mechanisms have not been well elucidated, restricting their potential clinical application. THP-1 is a human leukemia monocytic cell line, which has been used extensively to study monocyte/macrophage functions, mechanisms and signaling pathways [12], as well as the inflammation-modulating effects of food-derived compounds [13]. We isolated constituents of M. oleifera seeds and studied their effects on the expression of Th17-relevant cytokines using LPS-stimulated THP-1 cells. Considering that Th17-relevant cytokines are critically involved in the pathogenesis of psoriasis [14], we further evaluated their potential therapeutic application in psoriasis using a 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated mouse model
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
