Abstract
Rifampicin dependent mutants had been isolated as conditional lethal mutants with a lesion affecting transcription. Substituting Tn10 coded tetracycline resistance as a stable marker instead of the unstable rifampicin dependence facilitated mapping of dependence mutations. Three new rid loci were defined in this way. ridB and ridD were on opposite sides of ilv at about 84 min, and ridC was at about 35 min on the Escherichia coli chromosomal linkage map. Two more mutants with the previously described ridA lesion were also found. Growth response to removal of rifampicin was different for ridA, B, or C strains. Double mutants were constructed, and in all cases the more limiting phenotypic response prevailed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
