Théorie, principes et applications des techniques de fluorescence en biologie cellulaire et cancérologie

Abstract

Using fluorescent technologies under the microscope is becoming unavoidable in biology research laboratories. These technologies, stemming from instrumental developments of biophysics, raised with the use of fluorescent proteins derived from the GFP (green fluorescent protein) as well as the market development of commercial instruments. They allow studying the dynamics of molecules of interests (FRAP, FCS...) as well as their interactions (FRET, FLIM...) on cells, cellular extracts or tissues. Unfortunately, these are often used without detailed knowledge of their principles as well as their theories. These aspects are developed and illustrated here through means of literature example.