Abstract
A rhodamine B thiospirolactone-based probe, compound 1, namely 3′,6′-bis(diethylamino)-3H-spiro[2-benzothiophene-1,9′-xanthene]-3-one, has been successfully developed as a highly sensitive and selective fluorescent probe for imaging hypochlorous acid generated in human neutrophil cells. The probe was found to display “Off–On” switching in its fluorogenic and chromogenic responses to HClO and this was suggested to result from the hypochlorous acid-induced thio-spiro ring opening. This probe displayed excellent performance in high sensitivity, good selectivity for hypochlorous acid over other reactive oxygen species in biological pH condition, rapid reactivity as well as high tolerance to autoxidation under light. The probe has been successfully applied to detect HClO generated in living human neutrophil cells, implying the probe might be a new efficient tool in detecting the roles of hypochlorous acid in biological systems.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
