Abstract
Thiopurine prodrugs are antiviral chemicals used in medical therapy whose mechanisms of action are associated with inhibition of purine biosynthesis. In terms of plant chemotherapy, previous research of 6-mercaptopurine (MP) administration in tobacco tissue culture infected by Tobacco mosaic virus (TMV) showed no inhibition of virus activity. Currently, not enough data exist to confirm thiopurine drug ineffectiveness against viruses in the plant kingdom. This paper presents a screening of MP, 6-methylmercaptopurine riboside (MMPR), 6-thioguanine (6-TG) and 1-amino-6-mercaptopurine (1A-MP) against TMV and Cucumber mosaic virus (CMV) in in vitro tobacco explants and against Grapevine leafroll-associated virus 3 (GLRaV 3) in in vitro grapevine explants. ELISA and RT-PCR were used to evaluate antiviral activity. Higher toxicity levels of MP derivatives, compared to MP, were noted in tobacco and grapevine explants. 1A-MP or 6-TG treatment resulted CMV and GLRaV 3 virus-eradicated explants as obtained with Inosine 5′-monophosphate dehydrogenase inhibitors, whereas TMV was not eradicated by any of the studied drugs.
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