Thio-Michler's ketone, a new reagent for sulfenyl iodide and thiol proteins

Abstract

Summary Halogens oxidize thio-Michler's ketone (I) to an intensely blue disulfide (II), but sulfenyl iodides react with the thioketone (I) to give turquoise green mixed disulfides. The same turquoise colored products are formed from a thiol protein and the blue disulfide of thio-Michler's ketone (II). Both reactions can find use in studies of protein sulfenyl iodide derivatives and of thiol proteins. The high values of molar extinction of the colored compounds result in high sensitivity of the reactions.