Abstract
The photoactivable carbene precursor, 2-diazo-3,3,3-tri-fluoro-propionyloxy group, has been introduced recently for light-induced covalent crosslinking in studies of protein-phospholipid interactions in biomem-branes. The diazo group in this reagent has now been shown to undergo reduction in the dark by a number of thiols (dithiothreitol, 2-mercaptoethanol, cysteine and reduced glutathione) used commonly as protective agents for proteins. In contrast, thioglycolate did not cause significant reduction and, therefore, can be safely used as a protective agent. In vesicles formed from phospholipids containing the above photolabel in the fatty acyl chain, dithiothreitol and 2-mercaptoethanol caused reduction. However, cysteine and reduced glutathione caused insignificant reduction of the diazo group, presumably because of their non-permeant nature.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
