Abstract
Ribosomal RNA of E. coli contains some 16 4-thiouridylic acid residues per ribosome. These are not available for reaction in the 70S particle until it is dissociated into subunits. All the RNA and protein thiol groups then react with N-ethylmaleimide. Circular dichroism and melting profiles indicate that the 4-thiouridylic acid residues are in base-paired regions of the chain.
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