Abstract
Hydrogen peroxide (H2O2) is one type of reactive oxygen species (ROS) that can lead to a variety of forms of oxidative stress damage in human beings. Numerous methods have been used to detect H2O2 concentrations in various environments, however, these often suffer from inadequate detection limits, instrumental complexity, and multi-step experimental design, which may render them unfeasible for the required application. Herein, we report on a novel method for H2O2 detection that utilizes thiol-based SiOx nanodots (S-SiOx NDs) to initiate a sol-gel phase transition which can be observed by naked eye. This approach could lead to a very simple, rapid, and low cost method for H2O2 detection down to 5.8 μM, which is lower than the FDA regulation for H2O2 in food packaging. Furthermore, using a PL spectrometer allows H2O2 detection down to 0.01 μM. This S-SiOx NP system allows researchers the flexibility to choose between rapid visible detection of H2O2, or very high sensitivity detection by PL spectrometry.
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