Abstract
In the present research, water soluble thioglycolic acid-capped CdS quantum dots (QDs) were synthesized by chemical precipitation method. The characteristics of prepared quantum dots were determined using X-Ray Diffraction (XRD) and Transmission Electron Microscopy (TEM). The obtained results revealed that CdS QDs have 5.60nm crystallite size, hexagonal wurtzite structure and spherical morphology with less than 10nm diameter. The photoluminescence (PL) spectroscopy was performed in order to study the effect of the presence of starch solutions. Blue emission peaks were positioned at 488nm and its intensity quenched by increasing the concentration of starch solutions. The result of PL quenches in range of studied concentrations (0-100ppm) was best described by Michaelis-Menten model. The amount of Michaelis constant (Km) for immobilized α-amylase in this system was about 68.08ppm which showed a great tendency of enzyme to hydrolyze the starch as substrate. Finally, the limit of detection (LOD) was found to be about 2.24ppm.
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