Thinner Retinal Nerve Fiber Layer in Very Preterm Versus Term Infants and Relationship to Brain Anatomy and Neurodevelopment

Abstract

To assess retinal nerve fiber layer (RNFL) thickness at term-equivalent age in very preterm (<32weeks gestational age) vs term-born infant cohorts, and compare very preterm infant RNFL thickness with brain anatomy and neurodevelopment. Cohort study. RNFL was semi-automatically segmented (1 eye per infant) in 57 very preterm and 50 term infants with adequate images from bedside portable, handheld spectral-domain optical coherence tomography imaging at 37-42weeks postmenstrual age. Mean RNFL thickness was calculated for the papillomacular bundle (-15 degrees to+15 degrees) and temporal quadrant (-45 degrees to+45 degrees) relative to the fovea-optic nerve axis. Brain magnetic resonance imaging (MRI) scans clinically obtained in 26 very preterm infants were scored for global structural abnormalities by an expert masked to data except for age. Cognitive, language, and motor skills were assessed in 33 of the very preterm infants at 18-24months corrected age. RNFL was thinner for very preterm vs term infants at the papillomacular bundle ([mean ± standard deviation] 61 ± 17 vs 72 ± 13μm, P < .001) and temporal quadrant (72 ± 21 vs 82 ± 16μm, P= .005). In very preterm infants, thinner papillomacular bundle RNFL correlated with higher global brain MRI lesion burden index (R(2)= 0.35, P= .001) and lower cognitive (R(2)= 0.18, P= .01) and motor (R(2)= 0.17, P= .02) scores. Relationships were similar for temporal quadrant. Thinner RNFL in very preterm infantsrelative to term-born infants may relate to brain structure and neurodevelopment.