Abstract
Acid mucopolysaccharides in urines in Hunter's syndrome and in normal urine were fractionated by column chromatography and an enzymatic method with chondroitinase ABC. Molecular weight distributions of individual acid mucopolysaccharides were compared with each other by thin-layer gel filtration chromatography on Sephadex G-200, superfine. Approximate molecular weight of dermatan sulfate in Hunter's urine estimated by thin-layer gel filtration was 12000. Dermatan sulfate isolated from mature rat skin (approx.M.W. 21000) was degraded with testicular hyaluronidase to a material, the molecular weight of which was nearly equal to that of dermatan sulfate in Hunter's urine. Heparitin sulfate in Hunter's urine was monodisperse and low in molecular weight (approx. 5000). On the contrary, heparitin sulfate in normal urine was extremely polydisperse and heterogeneous. The molecular weight distribution of this material seemed to be much larger than that of other urinary acid mucopolysaccharides.
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