Thin-layer chromatographic studies of phenolic compounds in radiation-induced chlorophyll mutations in barley

Abstract

Using thin-layer chromatography, the spot patterns of phenolic compounds in young leaves of 32 chlorophyll mutations (26 radiation-induced, 2 chemically induced and 4 spontaneous) in barley, Hordeum vulgare L. were investigated. Out of a total of 29 spots, 14 were selected as marker spots. On the basis of differences in the spot pattern, the mutations were divided into four groups — one with a normal spot pattern and three with “mutant” spot patterns. The three latter groups differed distinctively from the “normal” one and from one another. Of the mutation types chlorina, viridis, xantha, and albina, only the last one was confined to one “mutant” group, whereas each of the viridis and xantha types was found in all three “mutant” groups, and the chlorina type was found in the “normal” group as well as in two of the “mutant” ones.