Thin layer iso-electro focusing of intracellular bacterial protein in classification of some representatives of the families Enterobacteriaceae and Vibrionaceae.

Abstract

Intracellular bacterial protein from some representatives of the families Enterobacteriaceae and Vibrionaceae has been investigated using thin layer iso-electric focusing in polyacrylamide gel. A marked difference between the protein patterns was observed between species within the same family. Carrying out the iso-electric focusing at pH range 3.5-9 gave a large number of protein bands. Increased resolution of the protein bands was obtained using iso-electric focusing at the pH range 4-6.5. Careful standardization of sample preparation, application and running conditions gave good reproducibility of intracellular proteins from bacterial subcultures.