Abstract
Intracellular bacterial protein from some representatives of the families Enterobacteriaceae and Vibrionaceae has been investigated using thin layer iso-electric focusing in polyacrylamide gel. A marked difference between the protein patterns was observed between species within the same family. Carrying out the iso-electric focusing at pH range 3.5-9 gave a large number of protein bands. Increased resolution of the protein bands was obtained using iso-electric focusing at the pH range 4-6.5. Careful standardization of sample preparation, application and running conditions gave good reproducibility of intracellular proteins from bacterial subcultures.
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