Abstract
Two efficient solvent systems are described for separation of phospholipids and lysophospholipids in one-dimensional thin-layer chromatography. A chloroform–methanol–acetic acid–acetone–water solvent system (35:25:4:14:2, v/v) was found to be suitable for separation of 10 phospholipids using a silica gel G plate. An chloroform–methanol–28% aqueous ammonia solvent system (65:35:8, v/v) also provided clear separation of major phospholipids and their lyso forms. An analysis of phospholipids from the CA1 region of rat hippocampal slices was achieved by separation of the above solvent systems and quantification of densitometric scanning chromatograms of specific-phosphate by staining with molybdenum blue reagent.
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