Abstract
The separation of biologically occurring non-volatile organic acids has been examined by thin-layer chromatography using a variety of solvent systems and thin-layer materials. The advantages of the two-dimensional method devised include the simultaneous demonstration of some keto acids as their oximes, sharp definition of the acidic spots, improved resolution over other published methods and rapid development. The method is very suitable for the biochemical diagnosis of known inborn errors of organic acid metabolism.
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