Abstract

The separation of eighteen corticosteroids and pregnane derivatives in submicrogram quantities by thin-layer chromatography is reported. Positional isomers, axial-equatorial hydroxyl epimers, and A/B cis-trans isomers were successfully resolved. Rapid separation of aldosterone, cortisone, and cortisol was achieved. Reversal of some relative mobilities was observed with two solvent systems.

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