Abstract
The thin-layer chromatography behaviour of cardenolides and their derivatives was investigated in the presence of boric acid, which forms cyclic derivatives with cis-1,2- and -1,3-diols. Boric acid reduces the mobility of cardenolide glycosides containing diol units in their carbohydrate moiety whereas with cardenolides or cardenolide glycosides possessing 1,3-diol units in the genin part it increases the mobility. The formation of boric acid derivatives resulted in an improved separation of certain cardiac steroids and afforded the possibility of detecting cardenolides containing reactive diol units. A method of impregnation in the vapour phase was developed which ensured mild treatment of the layers and yielded an extremely uniform impregnation.
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