Abstract
Conditions for the separation by thin-layer chromatography of the oximes, semicarbazones, 3-quinolylhydrazones and 2,4-dinitrophenylhydrazones of pyruvic acid, oxalacetic acid, and α-oxoglutaric acid, respectively, are described. The stabilities of the derivatives of oxalacetic acid have been compared with that of free oxalacetic acid by measuring the rate of spontaneous decarboxylation manometrically at pH values from 0.1 to 7.0. The oxime and dinitrophenylhydrazone derivatives are considerably more stable than the semicarbazone, quinolylhydrazone, or oxalacetic acid itself.
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