Abstract
Herbal medicinal products have a long-standing therapeutic record. To ensure the quality of herbal products specific identification tests which allow experts to discriminate related species and/or potential adulterants/substitutes are required. The purpose of the research was to recommend an original and simple method for the separation of closely related five triterpenic acids (ursolic, oleanolic, tormentic, euscaphic, pomolic acid) and its application to chemotaxonomy studies. 17 standard samples of Potentilla species and 3 test samples were chromatographed (with or without prechromatographic derivatization) on silica gel plates using the mobile phase: chloroform-diethyl ether-methanol-formic acid (30:10:1:0.2 v/v/v/v); they were subsequently derivatized, and visualized in UV 366 nm light. After images received pre-treatment (montaging stack, removal of noise, background subtract, horizontal equalization, two types of warping) exploratory analysis of the investigated Potentilla species fingerprints was processed. The method without prechromatographic derivatization was used to create differential fingerprints. Good results were obtained by the application of r-project iterative algorithm for subtract background and our simple algorithm for warping to image processing of TLC chromatograms for the first time.
Highlights
Herbal medicinal products have a long-standing therapeutic record and are still used as cure for various diseasesHow to cite this paper: Świeboda, R., Jóźwiak, A., Jóźwiak, G. and Waksmundzka-Hajnos, M. (2014) Thin Layer Chromatography and Chemometric Studies of Selected Potentilla Species
It is the quality of herbal medicinal products that is of major importance since chemical complexity of herbs varies greatly due to a wide range of factors such as species variation, hybrids, growth location and condition, climate, harvesting season and condition as well as drying and storage condition
The original Thin layer chromatography (TLC) images were exported from winCATs TLC workstation (CAMAG) to ImageJ (1.48c version), public domain, Java-based image processing program developed at the National Institute of Health
Summary
Herbal medicinal products have a long-standing therapeutic record and are still used as cure for various diseasesHow to cite this paper: Świeboda, R., Jóźwiak, A., Jóźwiak, G. and Waksmundzka-Hajnos, M. (2014) Thin Layer Chromatography and Chemometric Studies of Selected Potentilla Species. It is the quality of herbal medicinal products that is of major importance since chemical complexity of herbs varies greatly due to a wide range of factors such as species variation, hybrids, growth location and condition, climate, harvesting season and condition as well as drying and storage condition. To ensure the quality of herbal medicinal products, European Medicines Agency (EMA) suggest establishing good agricultural and collection practice for herbal starting materials (GACP) [1] and emphasize specific identification testing which allows experts to discriminate related species and/or potential adulterants/substitutes [2]. Thin layer chromatography (TLC) is frequently used as a chromatographic technique in fingerprint analysis of herbal medicines. TLC is considered to be a very convenient method in quality determination and in tracing possible adulteration of herbal products [4]
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