Abstract
Continuous solvent flow thin-layer chromatography with horizontal development using the BN-chamber was successful in the separation of isoquinoline alkaloids on this layers of silica gel H F 254 with acidic and basic solvent systems. Attempts to replace silica gel by other adsorbents did not have a desirable effect. Quantities as low as 1 μg of pure alkaloids were applied and detected by fluorescence under long-wave UV light. The quantity applied was under the detection limit of Dragendorff and iodoplatinate reagents. This procedure is limited to lesser quantities (a maximum of 5 μg of alkaloid per spot) owing to the overlapping of the various spots.
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