Thin layer chromatographic investigation of antibiotics residues in edible poultry tissues in Bangladesh

Md Shafiqul Islam Md Shafiqul Islam,Md Rakibul Hasan Md Rakibul Hasan,Md Shakil Islam Md Shakil Islam

doi:10.30574/wjbphs.2021.5.3.0019

Md Shafiqul Islam Md Shafiqul Islam, Md Rakibul Hasan Md Rakibul Hasan + Show 1 more

Open Access

https://doi.org/10.30574/wjbphs.2021.5.3.0019

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Abstract

Majority of the people in Bangladesh are still not aware about the health hazards of antibiotic residues. In this perspective, a survey was undertaken at five different poultry farms in the Dinajpur district. One hundred liver, one hundred thigh muscle and one hundred breast muscle samples were collected from different farms & market places and evaluated by TLC method. A very negligible number of samples were found positive for antibiotics residues. Out of one hundred liver, one hundred thigh muscle and one hundred breast muscle samples respectively; only two liver, two breast muscle and two thigh muscle samples were found positive for amoxicillin antibiotic; five liver, three breast muscle and three thigh muscle samples were found positive for ciprofloxacin; three liver, two breast muscle and two thigh muscle samples were found positive for cephalexin; two liver, two breast muscle and two thigh muscle samples were found positive for enrofloxacin; four liver, two breast muscle and two thigh muscle samples were found positive for oxytetracycline. Gentamicin and neomycin were found negative for any samples. Further investigation was done in indoor discriminate and indiscriminate use of antibiotics (ciprofloxacin and oxytetracycline) in broilers. Liver, thigh muscle and breast muscle samples were collected and evaluated by TLC method. Therefore, poultry treated with antibiotics are required for specific withdrawal period until all residues are depleted to safe levels before human consumption. This research project provides idea that Bangladeshi poultry products are free of antibiotics residues in north area (Dinajpur District) of Bangladesh.

Highlights

Poultry is one of the world’s quickest growing sources of meat, addressing nearly one-fourth of all the meat produced during the year 2000
Thin Layer Chromatography (TLC) analysis revealed that out of one hundred liver samples only two samples were positive for amoxicillin, three samples were positive for ciprofloxacin, four samples were positive for cephalexin, two samples were positive for enrofloxacin, two samples were positive for tetracycline and gentamicin & neomycin were found negative for any samples
In case of thigh muscles, out of one hundred samples, two samples were positive for amoxicillin, three samples were positive for ciprofloxacin, four samples were positive for cephalexin, two samples were positive for enrofloxacin, two samples were positive for tetracycline and gentamicin & neomycin were found negative for any samples

Summary

Introduction

Poultry is one of the world’s quickest growing sources of meat, addressing nearly one-fourth of all the meat produced during the year 2000. Advanced production unit can produce broiler chickens in less than six weeks This development arose from hereditary selection, improved feeding and health management practices. It involves usage of antibiotics as therapeutic agents to treat bacterial diseases in intensive farming systems [2]. Antibiotics are the most widely used veterinary drugs [14]. They are used by the poultry industry and veterinarians to improve growth rates, health, feed efficiency, egg production, or for therapeutic reasons and prophylaxis measures to reduce the incidence of poultry diseases [4,11]. Appearance of antibiotics residue in edible animal tissues remain a global problem [6]

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