Thin-layer chromatographic identification and gas-liquid chromatographic separation of seven aminobutyric acids in the presence of protein and non-protein amino acids

Abstract

Gas—liquid chromatographic separation of the following aminobutyric acids as their trifluoroacetyl n-propyl esters was achieved on 0.65% ethylene glycol adipate coated on a Chromosorb W AW: α-aminoisobutyric; α-aminobutyric; β-aminoisobutyric; β-aminobutyric γ-aminobutyric; γ-amino-β-hydroxybutyric and diaminobutyric acid. Only the two isomers of 3-aminobutyrate were not completely separated. Additionally, the positions of the aminobutyric acids and some other amino acids occurring in bacterial fermentation broths on a cellulose thin-layer plate after two-dimensional separation are given. In the fermentation broth of Clostridium oncolyticum M 55, only α-aminobutyric and γ-aminobutyric acid were identified as excretion products. In addition to these, Clostridium sporogenes produced additionally β-aminobutyric acid in quantities equalling γ-aminobutyric acid production. α-Aminoisobutyric and β-aminoisobutyric acid were completely absent, and γ-amino-β-hydroxybutyric and diaminobutyric acid were detected in low concentrations throughout the entire fermentation.