Abstract
Abstract TLC and HPLC techniques have been examined for histamine determination in fish. In case of HPLC determination a comparison of the performance characteristics of UV-Vis and Fluorescence detection is also described. Regression analysis was performed between the two sets of data, in order to examine the relative efficiency of the two techniques for the determination of histamine in fish sample extracts. Either of the two techniques proved sufficient for the determination of histamine in common concentration ranges in fish extracts although fluorescence detection had better sensitivity. The recovery of histamine from fish samples was decreased in comparison to the standard solutions but it was still sufficient for routine analysis of the fish samples.
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