Abstract
Carbon substrate films prepared by indirect evaporation have important features needed for the visualization of DNA in dark field work. These indirectly deposited carbon films can be made with minimum granularity and with a support thickness of about 10-20Å. They can mechanically withstand contact with aqueous solution and the high beam currents at condenser crossover in the microscope. The surface of freshly made carbon films used within a few hours wets and adsorbs DNA prepared by the Kleinschmidt method with its cytochrome c film. The low frequency binding on these carbon substrates even when fresh or after ultra-violet light activation makes them a marginal substrate film for DNA work (1,2). Unfortunately these films also lose their ability to wet and bind naked DNA or DNA spread with a basic protein within a period of a day.
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