Abstract
Calcium-induced-calcium-release (CICR) was assayed in unfertilized golden hamster eggs by injecting Ca 2+ and monitoring Ca 2+-dependent hyper-polarizing responses (HRs) and Ca 2+-sensitive fluo-3 fluorescence. Incubating eggs in the sulfhydryl reagent thimerosal caused [Ca 2+] 1 oscillations as monitored by Ca 2+-dependent HRs and decreased approximately 10-fold the Ca 2+ injection current required to generate an HR and cause a large intracellular Ca 2+ increase. Thimerosal also enhanced the sensitivity of eggs to Ca 2+ injection in a calcium-free medium. The effects of thimerosal on CICR were prevented by dithiothreitol and were not mimicked by injecting inositol 1,4,5-triphosphate. The data suggest that thimerosal may be an alternative agent for studying CICR in caffeine-insensitive cells.
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