Abstract

15Aug 2017 THIDIAZURON - INDUCED HIGH FREQUENCY SHOOT REGENERATION FROM LEAF AND NODE DERIVED CALLUS OF PUERARIA TUBEROSA DC, WILLD, A MEDICINALLY IMPORTANT WOODY CLIMBER. V. Sadguna and Md. Mustafa. Doctor of Philosophy in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India. Assistant Professor in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India.

Highlights

  • Pueraria tuberosa is widely spread over China, Japan, India and Pakistan

  • Till to date there is no proper protocol for the regeneration of plantlets for use. This we have developed a protocol for the high frequency regeneration of plantlets from leaf and node derived callus of Pueraria tuberosa by using TDZ and IAA in MS medium with repeated subcultures

  • Callus induction: leaf and node explants of Pueraria tuberosa were cultured on MS medium fortified with 2, 4-D, NAA and IAA alone and incombination with BAP

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Summary

RESEARCH ARTICLE

THIDIAZURON - INDUCED HIGH FREQUENCY SHOOT REGENERATION FROM LEAF AND NODE DERIVED CALLUS OF PUERARIA TUBEROSA DC, WILLD, A MEDICINALLY IMPORTANT WOODY. 1. Doctor of Philosophy in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana. 2. Assistant Professor in Botany, Department of Botany, Kakatiya University, Warangal, 506009, Telangana State, India

An efficient protocol is developed for the induction of shoot
In vitro shoot bud regenerarion

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