Abstract

Interpretation of radiation effects on living cells requires the determination of the absorbed dose. Dosimetry in cell monolayers irradiated by charged particles needs the knowledge of the mean cell thickness. Thickness measurement was made utilising the energy alteration of alpha particles traversing the cell monolayers. The reduced energy was determined from diameters or lengths of etched tracks produced by the penetrating alpha particles in CR-39. The experiments were performed using 3T3 cells grown directly on the CR-39 surface. The reliability of the method has been studied by means of a detailed error analysis. The total thickness errors are composed of the measurement uncertainty and the fluctuations of the cell thickness within the monolayers.

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