Thiamphenicol의 단일 경구투여에 따른 넙치와 조피볼락의 혈액내 잔류 분석

Abstract

넙치(<TEX>$700{\pm}50g$</TEX>, <TEX>$23{\pm}1.5^{\circ}C$</TEX>) 및 조피볼락(<TEX>$500{\pm}30g$</TEX>, <TEX>$23{\pm}1.5^{\circ}C$</TEX>)에게 Thiamphenicol(TP)을 1일 1회 경구(100 mg/kg BW) 투여한 다음, 경시적(1시간~432시간)으로 혈청 내 TP의 잔류량을 HPLC로써 분석하였다. TP를 어류 혈청에 0.1, 1.0, <TEX>$10{\mu}g/mL$</TEX>으로 첨가한 각각의 농도에 대하여 넙치 및 조피볼락에서 TP의 평균 회수율은 77.05~97.23%와 89.96~97.11%로 나타났다. TP의 경구 투여에 따른 넙치와 조피볼락의 체내 약물 혈중농도는 two-compartment model로 조사되었다. TP를 투여 후 넙치 혈청에서 10시간째 <TEX>$10.08{\mu}g/mL$</TEX>와 15시간째 <TEX>$10.06{\mu}g/mL$</TEX>로 최대값을 보였고, 조피볼락 혈청에서는 15시간째 <TEX>$8.88{\mu}g/mL$</TEX>로 최대값을 나타내었다. 넙치와 조피볼락의 혈청에서 TP는 투여 후 432시간째(18일째) 모든 시료에서 검출한계 이하로 검출되지 않았다. TP의 어류 체내 잔류 양상은 넙치와 조피볼락에서 매우 유사하였다. 본 연구에서 얻어진 결과는 넙치와 조피볼락에 TP를 처방하여 치료 계획을 수립할 때 유용하게 활용될 것으로 여겨진다. This study was aimed at investigating the residual patterns of thiamphenicol (TP) in the cultured olive flounder (Paralichthys olivaceus) and black rockfish (Sebastes schlegeli) following oral administration. TP concentration were detected by high performance liquid chromatography with UV detector. The recovery rates of TP in serums ranged 77.05~97.23% (olive flounder) and 89.96~97.11% (black rockfish) for the concentration of 0.1, 1.0, <TEX>$10{\mu}g/mL$</TEX>, respectively. After single administration of TP (100 mg/kg body weight) by oral route in olive flounder (<TEX>$700{\pm}50g$</TEX>, <TEX>$23{\pm}1.5^{\circ}C$</TEX>) and black rockfish (<TEX>$500{\pm}30</TEX><TEX>g$</TEX>, <TEX>$23{\pm}1.5^{\circ}C$</TEX>), the concentration in the serum was determined at 1, 5, 10, 15, 24, 30, 50, 168, 264 and 432 h post-dose. Two-compartment model was applied to analyze in the pharmacokinetics of TP administered to the fishes. In the serum of olive flounder, TP was detected on 10 and 15 hours after treatment were <TEX>$10.08{\mu}g/mL$</TEX> and <TEX>$10.06{\mu}g/mL$</TEX> as maximum level, respectively. In the serum of black rockfish, TP was detected on 15 hours after treatment were <TEX>$8.88{\mu}g/mL$</TEX> as maximum level. Concentrations of TP to the fishes were not measurable at 432 hours (18 days) after treatment in all samples. Similar residual patterns of TP were revealed between the fishes. These results are helpful for estimating withdrawal time of TP which has been already in use for farmed fish treatment.