Abstract
The study of factors altering the CNS and GI absorption of thiamine. In rats required the development of a specific assay for thiamine from 100-μl samples of blood and plasma and small quantities of tissue. The specific thiochrome fluorescence assay for thiamine was modified to handle microsamples and to use 35S-thiamine. This sensitive and specific radioassay using 35S-thiamine gave pharmacokinetic parameters for 4-mg/kg iv doses of thiamine in rats equivalent to those using the less sensitive thiochrome fluorescence assay. The new assay, because of its lower limit of detection, allowed the study of the time profile of thiamine after a 1-mg/kg iv dose in rats. Such a time profile could not have been followed using the standard thiochrome fluorescence assay.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
