Abstract
Bioautography has been used to identify the thiamine-active substances accumulated in Neurospora mycelia and culture media of wild type and thi-1 strains. These strains have been compared at different stages of growth to detect differences in thiamine-active compounds. The nature of the thi-1 block has been investigated. The bioautographic characteristics of thiamine-active substances from thi-1 strains were indistinguishable from those of thi-1 + strains in these experiments. Thiamine phosphates are nutritionally relatively unavailable to thi-1 strains. The presence of high levels of thiamine phosphates in extracts of thi-1 mycelia explains the poor growth of thi-1 strains on these extracts. Thiamine mono-, di-, and triphosphates have been identified by their properties in two chromatographic systems and auxanographic assays by three mutant strains. The main component in these extracts is thiamine diphosphate.
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