Abstract
1. The nutrient solution used containing mineral salts and pure dextrose does not contain any appreciable amount of growth-promoting substances. 2. Pythium arrhenomanes, P. deliense, P. graminicolum, P. hyphalosticton andP. mamillatum are capable of unlimited growth in the nutrient solution. 3. The results of the experiments show that the organisms are capable of synthesising their own growth-promoting substance from the elementary ingredients of the nutrient solution. 4. Some of the growth-promoting substance manufactured by the organisms is also given off by the mycelium into the solution. 5. The addition of thiamin to nutrient medium B has no marked effect on the growth of these organisms. 6. The failure ofPythium arrhenomanes andP. hyphalosticton to grow in the synthetic liquid medium used by Robbins and Kavanagh is found to be due to the concentration of the medium. It is suggested that the concentration hinders the synthesis of growth-promoting substance and thus affects the growth.
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