Thiamethoxam-induced hematological, biochemical, and genetic alterations and the ameliorated effect of Moringa oleifera in male mice

Abstract

Thiamethoxam (TMX) exerts pronounced insecticidal effects against a wide variety of economically imperative pests. However, the administration of TMX in experimental animals induced notable adverse effects on the function of various organs. The purpose of this study was to assess TMX induced hematological, biochemical, and genetic alterations and the potential ameliorative effects on them of Moringa oleifera leaf extract (MLE) in male mice Animals were orally administered TMX (≈1/10 LD50) daily either alone or with MLE (200 mg/kg b.w.) for 28 successive days. Blood was collected to evaluate the hematological profile and serum levels of Aspartate Aminotransferase (AST), Alanine aminotransferase (ALT), alkaline phosphatase (ALP), albumin, creatinine, uric acid, and urea. Liver and kidney cells were used to assess the Malondialdehyde (MDA) content and antioxidant enzymes. DNA integrity was estimated also in the liver and kidney using comet and colorimetric diphenylamine assays. Results revealed that TMX exhibited significant changes in the hematological profile and liver and kidney functions. Besides, TMX significantly raised the MDA content and DNA damage in both two of these organs. In contrast, TMX reduced the antioxidant activities in the cells of both liver and kidney. Meanwhile, Moringa extract combined with TMX significantly attenuated the deleterious findings of TMX. Specifically, it improved the TMX-induced hematological changes, liver and kidney function alterations, oxidative stress, and DNA damage rate. It can be concluded that TMX had adverse effects on different cells of male mice, but MLE successfully ameliorated TMX’s hematological and hepatorenal toxicity.