Thesensitization of mucosal mast cells during infections with Trichostrongylus colubriformis or Haemonchus contortus in sheep

Abstract

Responses of isolated mucosal mast cells (MMC) during infections with either Trichostrongylus colubriformis or Haemonchus contortus were examined by measuring the release of sheep mast cell protease (SMCP) in a degranulation assay. MMC from sheep immune to T. colubriformis released maximal amounts of SMCP and histamine within 0.5h of incubation with larval antigen whereas maximum secretion of leukotrienes occurred 3h after addition of antigen. It was only after 8 weeks of a primary T. colubriformis infection, that MMC released significantly elevated levels of SMCP (23%); this occurred when the worm burden was being rejected. In contrast, the SMCP release from MMC of immune sheep was significantly higher at 40%, and occurred within 1–4 days after challenge (DAC). The SMCP release peaked at 6–8 DAC at 51%, and declined after 56 DAC to <25%. MMC isolated from the duodenum and mid-small intestine of immune sheep released 2–3 times higher proportion of SMCP than did cells recovered from the terminal ileum. Mast cell numbers were similar in the 3 regions but the quantity of globule leucocytes (GL) was 2.5 times higher in the duodenum. During infections with H. contortus in the abomasum, MMC isolated from the small intestine released greater levels of SMCP when incubated with larval antigens than did abomasal MMC. There was no increased release during the first 12 weeks of a primary infection although the SMCP release (23%) from immune MMC at 7–10 DAC was significantly enhanced. Once again the release from MMC isolated from the three intestinal regions of sheep immune to H. contortus was lowest in the terminal ileum. Mast cell numbers were 2 and 3 times higher in the abomasum and duodenum than at the 2 distal sites and a significant number of GL was found only in the abomasum. The results suggest that the sensitization of MMC to parasite antigen during primary infection is closely associated with the development of acquired immunity to either parasite. The response is disseminated throughout the small intestine, and the high levels of SMCP release and number of GL at the site of parasitism probably reflect the greater sensitization of MMC in those region.