Abstract
Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases. High productivity values (t =1 hr) were obtained while completely avoiding adenosine-deaminase degradation of the products. N-2-deoxy-ribosyltransferase activity is described for the first time in hyperthermophilic bacteria.
Highlights
Thermophiles are a group of microorganisms that grow under extreme temperature conditions
These results seem to indicate that one very active nucleoside 2’-deoxyribosyltransferase or one thymidine nucleoside phosphorylase plus one purine nucleoside phosphorylase are present in these strains
If the strains: i) gave better yields versus adenine than with hypoxanthine; ii) gave better yields with 2’-deoxyribose nucleoside than with ribose nucleoside; iii) gave lower or similar yields using thymidine than using 2’-deoxyuracil, as sugar donors and iv) uridine is recognised as substrate, we could postulate that the strains present an active nucleoside 2’-deoxyribosyltransferases (NdRTs) or one PyNP plus one PNP selective versus adenine compared to hypoxanthine
Summary
Thermophiles are a group of microorganisms that grow under extreme temperature conditions.These microorganisms offer useful enzymes to expand the range of reaction conditions suitable for biocatalysis. Several mesophile microorganisms have been identified as active for purine nucleoside synthesis, giving relatively good yield values after short reaction times [5,6]. Thermus thermophilus strains - cultured in optimum conditions - were tested.
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