Abstract
ABSTRACTTwenty five thermotolerant yeasts isolated from different dairy products were tested for their ability to grow on lactose containing media and to produce β-galactosidase enzyme with enhanced transgalactosylation activity. It was confirmed that the most effective strains are those belonging to the genus Kluyveromyces. Two K. marxianus strains - 904 and t3 with relatively high specific activity of β-galactosidase (480 and 500 U/mg protein, respectively) were selected. It was shown that these strains possess a high yield of lactose transformation (hydrolysis and transgalactosylation) (98%) and high tri- and tetra- galacto-oligosaccharides (GOS) yield. More than 50% conversion rate of lactose to GOS was achieved when K. marxianus 904 and t3 were used as whole cell biocatalysts. The composition of the synthesized GOS was determined to be exclusively consisted of galactosyl residues.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
