Abstract
The biotransformation of isonicotinamide was investigated using thermophilic intracellular amidase produced from Geobacillus subterraneus RL-2a. Various process parameters, including amount of biocatalyst, substrate feeding rate, enzyme-to-substrate ratio and operational thermostability were systematically examined with the aim of achieving complete substrate conversion and high productivity. In 1L fed batch reaction containing 0.1M isonicotinamide, in 0.2M potassium phosphate buffer (pH 6.5, 200rpm) and 8Uml−1 amidase activity (12.48mgdcwml−1) of whole cells of G. subterraneus RL-2a (as biocatalyst) resulted in a yield of 0.1M of isonicotinic acid after 50min reaction time at 70°C and a total of 61.55g isonicotinic acid was produced at a rate of 1.18gh−1g−1dcw respectively. The volumetric productivity was 14.8gh−1l−1.
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