Abstract
Thermospray (TSP) liquid chromatography mass spectrometry and electrospray (ESP) mass spectrometry were applied to the analysis of the flavocoenzymes riboflavin (Rfv), riboflavin 5'-monophosphate (FMN) and flavin adenine dinucleotide (FAD). Positive and negative ion spectra are presented. TSP spectra of Rfv and FMN were characterized by the presence of intense and characteristic fragment ions containing the flavin ring whereas in those from FAD, ions containing the adenine moiety predominate. ESP affords mainly molecular weight information as well as some indication of the number of acidic places in these molecules. Application of these techniques to the analysis of root extracts from iron-depleted sugar beet ( Beta vulgaris) was instrumental in the characterization of two novel riboflavin compounds, riboflavin 3'-monosulphate (3-FMS) and riboflavin 5'-monosulphate (5-FMS), as the major flavins in roots.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.