Abstract
To assess the LC/MS methods for the determination of these compounds in biological materials positive ion mass spectra were measured for benzodiazepines and their metabolites by thermospray-liquid chromatography/mass spectrometry (TSP-LC/MS) and atomospheric pressure chemical ionization-liquid chromatography/mass spectrometry (APCI-LC/MS). In both TSP-LC/MS and APCI-LC/MS, LC analyses were performed on a TSKgel octyl-80Ts in the solvent system of methanol-ammonium acetate at a flow rate of 1.0 ml/min. TSP mass spectra showed only a [M+H]+ ion for most of compounds, while APCI mass spectra showed [M+H]+ and some fragment ions. The analysis of these compounds with TSP gave better detection limits than with APCI by scan mode, and gave the same results for detection limits as with APCI by selected ion monitoring (SIM). The data, described in this paper, should serve as a basis for the qualitative and quantitative analyses of benzodiazepines and their metabolites in biological samples.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
