Thermosonication: A technique to inactivate the plasmin system in milk

Abstract

This study investigated the synergistic effect of sonication and heat on plasmin, plasminogen-derived and plasminogen activators activity in buffalo milk. Sonication of milk samples at 72 ± 2 °C, 20 kHz, at 140 W from 60 to 150 s, indicated the activity of plasmin-system remained stable on sonication at 75 and 85% amplitude. On the other hand, increasing the exposure of milk to 95% amplitude decreased the enzymatic activities of plasmin, plasminogen-derived and plasminogen activators significantly when compared to heating without sonication. Thus, thermosonication can be applied to inactivate the plasmin-system in milk.