Abstract
We build thermoresponsive phospholipid-based artificial cell model compartments that respond to mild temperature changes in the environment by performing reversible shape deformations, enabling material transfer between entities, and migration. The temperature increase is in the laboratory applied by means of an IR-B-laser, and induces the self-organization of lipid compartments into dynamic clusters. The clusters are motile and can migrate along the temperature gradient. To increase the thermoresponsiveness, we encapsulate poly-N-isopropylacrylamide (PNIPAAm) in the lipid compartments. The polymer solution undergoes reversible gel formation when heated above its lower critical solution temperature (LCST) of 32 °C. We observe the formation of microaggregates inside the lipid compartments which can interact with the membrane and induce membrane deformations. Our experimental system comprises a contactless method to generate compartmentalized multicellular model structures with thermoresponsive properties.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
