Abstract
Ergovaline (EV) was isolated from Acremonium coenophialum-infected tall fescue seed. Seed (2 kg) was extracted with 20 L of 5% lactic acid in water/methanol (4:1), filtered, and passed over an Amberlite XAD-2 column. Ergovaline was eluted with MeOH, concentrated, and chromatographed on two reversed-phase C 18 columns (purity >95%, 40% overall recovery). It was administered to rats (15 μg/kg of BW, ip) at 22 o C to determine time-related acute effects on thermoregulatory ability
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