Thermolabile xylanase of the Antarctic yeast Cryptococcus adeliae: production and properties.

Abstract

Xylanase production by the Antarctic psychrophilic yeast Cryptococcus adeliae was increased 4.3 fold by optimizing the culture medium composition using statistical designs. The optimized medium containing 24.2 g l(-1) xylan and 10.2 g l(-1) yeast extract and having an initial pH of 7.5 yielded xylanase activity at 400 nkat (nanokatal) ml(-1) after 168-h shake culture at 4 degrees C. In addition, very little endoglucanase, beta-mannanase, beta-xylosidase, beta-glucosidase, alpha-L-arabinofuranosidase, and no filter paper cellulase activities were detected. Among 12 carbon sources tested, maximum xylanase activity was induced by xylan, followed by lignocelluloses such as steamed wheat straw and alkali-treated bagasse. The level of enzyme activity produced on other carbon sources appeared to be constitutive. Among the complex organic nitrogen sources tested, the xylanase activity was most enhanced by yeast extract, followed by soymeal, Pharmamedia (cotton seed protein), and Alburex (potato protein). A batch culture at 10 degrees C in a 5-1 fermenter (3.5-1 working volume) using the optimized medium gave 385 nkat at 111 h of cultivation. The crude xylanase showed optimal activity at pH 5.0-5.5 and good stability at pH 4-9 (21 h at 4 degrees C). Although the enzyme was maximally active at 45 degrees - 50 degrees C, it appeared very thermolabile, showing a half-life of 78 min at 35 degrees C. At 40 degrees - 50 degrees C, it lost 71%-95% activity within 5 min. This is the first report on the production as well as on the properties of thermolabile xylanase produced by an Antarctic yeast.