Abstract

A comparison was made for HF and M strains of herpes simplex virus for their thermosensitivity in various conditions. The infectivity of herpes viruses were preserved well by 10% bovine serum and 1% skim milk in PBS at 45°C for 10 minutes. The titers of HF and M strains showed pretty well preservations by PBS, MLE and 10% glycerin in PBS. The thermostabilizing effects of 10% DMSO, 1 M MgCl2, 1 M Na2SO4 in PBS and distilled water were fairly inferior. The thermostability of M strain under the various conditions was greater than HF strain. The range of optimal pH for high thermostability was more comprehensive in M strain as compared to HF strain.

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