Abstract
The thermodynamics associated with the Mn 2− · ATP, Mn · ADP − and Mn · AMP complex formation reactions determined from K potentiometric measurements at I = 0.2 are reported for the temperature range 1–45°C. The K values increase with the lenght of the phosphate chain and with temperature. The limits and the best conditions for use of the “pH stat” titration method are discussed. Comparison with the results obtained by potentiometric and calorimetric methods in the case of Mg · nucleotide complexes is made.
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