Abstract
The aim of this study was to investigate the nylon membrane’s characteristics and thermodynamic aspects for adsorption of Bovine Serum Albumin (BSA) as a model protein on nylon membrane. The morphology characteristics analyzed by FESEM and wetting and weighting technique showed the nylon membrane possessed micro-pore size (0.159±0.039 µm) with high porosity (74.2820±0.0411 %) respectively. Thermodynamic studies indicated that the adsorption reaction was endothermic with positive value of standard enthalpy (∆rHθ = 107.7 kJ/mole) and dominated by chemical adsorption. The results were supported with the analysis of functional groups of the nylon membrane with FTIR-ATR. Bands corresponding to the NH bonds stretching were generally detected in the 3500-3100 cm-1 range. The existence of C=O stretching vibration of carbonyl group was further confirmed with the observation of peak at 1632. 42 cm-1. The water contact angle analysis showed the hydrophilicity nature of nylon membrane with contact angle of 55.6°. These findings are expected to be used in the modification of nylon membrane for an optimum adsorption of protein.
Highlights
Biomaterial is defined as a material placed in contact with biological fluids and it is expected to function with a desirable host response in specific applications, without any desirable effects
It was observed that nylon membrane possessed a symmetrical structure with almost identical morphology
Nylon membrane was a symmetrical membrane with micro-pore size range
Summary
Biomaterial is defined as a material placed in contact with biological fluids and it is expected to function with a desirable host response in specific applications, without any desirable effects. Some examples of the usage of membrane as biomaterial in biomedical field are hemodialysis [3], bone regeneration [4] and diagnostic-kit [5]. In the latter, diagnostic-kit are favorable in overcoming the issues of scarcity of medical resources such as highly trained specialists and advanced diagnostic equipment. The assembly of the diagnostic-kit can be divided into two parts; the immunoagents extraction and membrane selection Membranes such as nitrocellulose [7], poly (vinylidenefluoride) [8], nylon [9] and cellulose acetate [10] have been used in the preparation of diagnostic-kit. It is important to analyze some insights about the interaction between the protein molecule and membrane interface via thermodynamic studies
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