Abstract
In this work, accelerated start-up of biological hydrogen production system fed with glucose and molasses at 55 °C by regulating pH and COD concentration was investigated in two groups. Then three reactors in each group were compared: controlling pH, controlling pH with COD, and controlling the COD. The reactors in group A presented best hydrogen yield of 1.84 mol H2/mol glucose·day and worked stably at the 8th day. The highest hydrogen yield in group B was 2.13 mol H2/mol molasses·day and steadily at the 11th day. It proved that controlling two key parameters of the inflow pH (8.0) and substrate concentration (4000 mg COD/L) could realize fast start-up of hydrogen production reactor. This study demonstrated that Thermoanaerobacterium sp. strain RBIITD could produce hydrogen and provide a new avenue for biological hydrogen production by dark fermentation using cheap substrate towards a more sustainable and feasible technology.
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